A PCR-based Test for the Specific Identification of Mycoplasma Mycoides Subspecies mycoides SC
نویسندگان
چکیده
منابع مشابه
Mycoplasma mycoides subsp. mycoides SC identification by PCR in sperm of seminal vesiculitis-affected bulls.
In this study, by using the polymerase chain reaction (PCR) diagnosis for the detection and identification of Mycoplasma, we investigated mycoplasmas contaminating the semen of yearling bulls affected by seminal vesiculitis. The bulls presented neither subclinical nor clinical contagious bovine pleuropneumonia signs and the complement fixation test for specific antibodies was negative. Furtherm...
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Rapid and specific detection of Mycoplasma mycoides subsp. mycoides Small Colony (M. mycoides SC) is important for the effective control of contagious bovine pleuropneumonia. Although the United States has been free of this disease for over 100 years, it is necessary to develop modern diagnostic assays that are sensitive and specific for biological agents that would affect the US agricultural i...
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Mycoplasma mycoides subsp. capri and Mycoplasma mycoides subsp. mycoides LC can be combined into one taxon on the basis of several contributions on both DNA sequence and protein analyses reported in the literature. Moreover, for the differentiation and identification of mycoplasmas of the "mycoides cluster", we investigated the rpoB gene, encoding the beta-subunit of the RNA polymerase. A segme...
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An outbreak of contagious bovine pleuropneumonia (CBPP) was investigated in the Somali National Regional State, Eastern Ethiopia, to isolate and identify the causative Mycoplasma species. Detailed physical clinical examination was performed on over 700 affected animals to select acutely sick animals for autopsy and bacteriological specimen collection. Postmortem examination and sample collectio...
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Acrylamide gel electrophoresis was used to show the similarities and differences in the membrane proteins of two vaccine and two virulent strains of Mycoplasma mycoides var. mycoides. Immunoelectrophoretic (IEP) analysis was also used to partially characterize the associated antigens. Antibody spectra to the antigens of M. mycoides differ in rabbit, pig, and cattle sera. Rabbits produce better ...
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ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 1994
ISSN: 1040-6387,1943-4936
DOI: 10.1177/104063879400600405